Label-free field-effect-based single-molecule detection of DNA hydridization kinetics
نویسندگان
چکیده
1. Fabrication of devices 2. Lifetime histograms with probe DNA NH2-5’-GGAAAAAAGG-3’ (Device 1) 3. Measurements with probe DNA NH2-5’-GTGAGTTGTT-3’ (Device 2) 4. Lifetime histograms with probe DNA NH2-5’-GTGAGTTGTT-3’ (Device 2) 5. Control experiment with a non-functionalized device 6. Scanning gate microscopy (IV) 7. Control experiments with probe DNA NH2-5’-GGAAAAAAGG-3’ with complementary and non-complementary target DNAs, respectively 8. Melting curves with UV-Vis 9. Noise 10. Bandwidth of nanotube device 11. Ergodicity 12. Kinetic modes
منابع مشابه
Label-free single-molecule detection of DNA-hybridization kinetics with a carbon nanotube field-effect transistor.
Single-molecule measurements of biomolecules can provide information about the molecular interactions and kinetics that are hidden in ensemble measurements. However, there is a requirement for techniques with improved sensitivity and time resolution for use in exploring biomolecular systems with fast dynamics. Here, we report the detection of DNA hybridization at the single-molecule level using...
متن کاملA novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes
Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-co...
متن کاملLabel-free free-solution nanoaperture optical tweezers for single molecule protein studies.
Nanoaperture optical tweezers are emerging as useful label-free, free-solution tools for the detection and identification of biological molecules and their interactions at the single molecule level. Nanoaperture optical tweezers provide a low-cost, scalable, straight-forward, high-speed and highly sensitive (SNR ∼ 33) platform to observe real-time dynamics and to quantify binding kinetics of pr...
متن کاملAn aptameric graphene nanosensor for label-free detection of small-molecule biomarkers.
This paper presents an aptameric graphene nanosensor for detection of small-molecule biomarkers. To address difficulties in direct detection of small molecules associated with their low molecular weight and electrical charge, we incorporate an aptamer-based competitive affinity assay in a graphene field effect transistor (FET), and demonstrate the utility of the nanosensor with dehydroepiandros...
متن کاملLabel-free optical detection of single enzyme-reactant reactions and associated conformational changes
Monitoring the kinetics and conformational dynamics of single enzymes is crucial to better understand their biological functions because these motions and structural dynamics are usually unsynchronized among the molecules. However, detecting the enzyme-reactant interactions and associated conformational changes of the enzyme on a single-molecule basis remains as a challenge to established optic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010